Microsatellite
Microsatellites are normally found in non-coding regions of DNA such as introns and intergenic sequences and mainly used as ‘neutral’ genetic markers
Microsatellite markers are co-dominant, multi-allelic, easily detected, hyper-variable, highly reproducible and abundant in the genome,
Applications include;
Variety identification, genetic diversity evaluation, phylogenetic analysis, genetic map construction, comparative mapping etc.
Most importantly, it detects a much higher level of DNA polymorphism than any other marker system
Bamboo has polymorphic DNA so highly polymorphic markers are required for mapping and analysis of its genome also known as; simple sequence repeats (SSRs), variable numbers of tandem repeats (VNTRs) and short tandem repeats (STRs).
The Bamboo Microsatellite is web based relational database of microsatellites present in whole genome sequence. Bamboo microsatellite allows microsatellite search using parameters like simple and compound repeats. Microsatellite can be retrieved by specifying the repeat types further these nucleotide sequence marker are also provided to facilitate primer designing using Primer 3 tool for PCR amplification of desired microsatellite.
Bamboo Microsatellite is available at http://14.139.223.245/bmDB